Coding

Part:BBa_K4395007:Design

Designed by: Ling Jiajun   Group: iGEM22_SYSU-CHINA   (2022-09-27)


SpyTag-RsAs-SpyCatcher


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The idea of fusing SpyTag and SpyCatcher on the N-terminus and C-terminus, respectively, on the enzyme RsAs enables the fusion protein to catalyze glucosyl transfer in higher temperature than one where the reaction normally happens.


Source

The SpyTag/SpyCatcher system is a derivative of CnaB2 domain of Streptococcus pyogenes. RsAs comes from the genome of Rauvolfia serpentina.

References